Detection of Thelohania solenopsae (Microsporidia: Thelohaniidae) in Solenopsis invicta (Hymenoptera: Formicidae) by multiplex PCR.

نویسندگان

  • Steven M Valles
  • David H Oi
  • Omaththage P Perera
  • David F Williams
چکیده

Oligonucleotide primer pairs were designed to unique areas of the small subunit (16S) rRNA gene of Thelohania solenopsae and a region of the Gp-9 gene of Solenopsis invicta. Multiplex PCR resulted in sensitive and specific detection of T. solenopsae infection of S. invicta. The T. solenopsae-specific primer pair only amplified DNA from T. solenopsae and T. solenopsae-infected S. invicta. This primer pair did not produce any amplification products from DNA preparations from uninfected S. invicta, seven additional species of microsporidia (including Vairimorpha invictae), or Mattesia spp. The Gp-9-specific primers recognized and amplified DNA from Solenopsis xyloni, Solenopsis richteri, Solenopsis geminata, the invicta/richteri hybrid, and monogyne and polygyne S. invicta, but not from T. solenopsae, and, as such, served as a positive control verifying successful DNA preparation. Multiplex PCR detected T. solenopsae in worker fire ants infected with as few as 5000 spores. Furthermore, multiplex PCR detected T. solenopsae in all developmental stages of S. invicta. However, detection could be made more sensitive by using only the T. solenopsae-specific primer pair; ants infected with as few as 10 spores were able to be discerned. Multiplex PCR detection of T. solenopsae offers the advantages of a positive control, a single PCR amplification, detection of all developmental stages, and increased sensitivity and specificity compared with microscopy.

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منابع مشابه

Long-Term Studies of the Red Imported Fire Ant, Solenopsis invicta, Infected with the Microsporidia Vairimorpha invictae and Thelohania solenopsae in Argentina

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Impact of Thelohania solenopsae (Microsporidia: Thelohaniidae) on polygyne colonies of red imported fire ants (Hymenoptera: Formicidae).

Three studies were conducted to assess the effects of the entomopathogen Thelohania solenopsae on polygynous, red imported fire ant, Solenopsis invicta Buren, colonies. A total of 57 of 122 queens (46.7%) from nine, field-collected, polygyne, S. invicta colonies, was infected with T. solenopsae. Infection rate of queens for each colony ranged from 25 to 75%. Laboratory colonies of polygyne S. i...

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Evidence of intracolony transmission of Thelohania solenopsae (Microsporidia: Thelohaniidae) in red imported fire ants (Hymenoptera: Formicidae) and the first report of spores from pupae.

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BIOLOGICAL CONTROLÐMICROBIALS Prevalence of Thelohania solenopsae (Microsporidia: Thelohaniidae) Infection in Monogyne and Polygyne Red Imported Fire Ants (Hymenoptera: Formicidae)

We determined the prevalence of natural Þeld infections of the Þre ant pathogen Thelohania solenopsaeKnell, Allen, and Hazard in the monogyne and polygyne social forms of the red imported Þre ant, Solenopsis invicta Buren, in three pastures in Florida. Social form was determined by examining the genotype of ants at the Gp-9 locus. The monogyne form contains a single fertilized queen per colony,...

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عنوان ژورنال:
  • Journal of invertebrate pathology

دوره 81 3  شماره 

صفحات  -

تاریخ انتشار 2002